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Physics of Life 2025 is large-scale conference representing the wide diversity of the UK biological physics community, continuing the biennial series started with Physics of Life 2023. Organised jointly by PoLNet3, IOP Biological Physics, the British Biophysical Society, Physics of Living Matter and Physics meets Biology, this in-person meeting will project the excitement of science at the interface between physics and biology. In addition to exciting keynote lectures, the conference will feature parallel sessions on a full breadth of topics, with many contributed talks, ensuring a wide focus and inclusion of the most cutting edge science.